Transfection efficiency of Lentivirus carrier to Schwann cells in vitro

doi:10.3969/j.issn.2095-4344.2014.51.019

Abstract

Abstract:

BACKGROUND: The Lentivirus carrier studied in recent years has been widely used in the gene transfection experiment due to its strong transfection ability and high transfection efficiency.
OBJECTIVE: To study the transfection efficiency of Lentivirus carrier to primary Schwann cells in vitro.
METHODS: The virus vector was constructed with Lentivirus three-plasmid system. In vitro, the primary Schwann cells were transfected by recombinant virus with different-multiplicities of infection (1, 5, 10, 20 and 40). At 1, 3, 5, 7 and 9 days after transfection, fluorescent expression of Lentivirus was observed under fluorescence microscope, and the transfection efficiency was calculated in the counting squares of the microscope. Green fluorescence presented the transfected Schwann cells, and the others were untransfected Schwann cells. Then the transfection efficiency was calculated.
RESULTS AND CONCLUSION: At 3 days after transfection, the small amount of fluorescence could be seen in the culture dishes with different multiplicities of infection, and then the fluorescence amount was increased at 5 days after transfection. It reached peak after 7 days. There was no significant difference in the fluorescence amount between 7 and 9 days after transfection. Different multiplicities of infection could lead to different transfection efficiencies: the transfection efficiency was 45% when the multiplicity of infection was 1; the transfection efficiency was 80% when the multiplicity of infection was 5; the transfection efficiency was 90% when the multiplicity of infection was 10; the transfection efficiency was 78% when the multiplicity of infection was 20; and the transfection efficiency was 70% when the multiplicity of infection was 40.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

全文链接：

Key words: lentivirus, schwann cells, transfection, cells, cultured

CLC Number:

R318

Lian Xiao-feng, Xu Jian-guang, Zeng Bing-fang, Zhou Wei, Kong Wei-qing, Zhang Tao, Hou Tie-sheng. Transfection efficiency of Lentivirus carrier to Schwann cells in vitro[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(51): 8301-8304.

Figures/Tables 3

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